Case study: Canine non‐epitheliotropic CD4‐positive cutaneous T‐cell lymphoma: a case report
Robert Lo, Ph.D, D.V.M
A 5‐year‐old, spayed female French Bulldog presented with multiple papules on the skin of the scapular area. Histopathological examination of skin biopsy specimens showed proliferated small lymphoid cells in the superficial dermis and in the area around the hair follicle. Immunohistochemical examination revealed that these cells were positive for CD3, CD4 and TCRαβ antibodies, but negative for CD1c, CD8α, CD8β, CD11c, CD20, CD45RA, CD90, MHC-II and TCRγδ antibodies. In addition, CD45 is highly expressed, and proliferation is very low. The genetic recombination test of the T cell receptor G chain detects the proliferation of recombinant clones. Skin lesions were removed by surgery because of progressing to the outside of the forelegs. The postoperative clinical course was good, and no recurrence was observed until the dog died in a traffic accident about a year later.
Figure 1 Clinical features of the dog. Multiple papules are present on the right scapular area.
Figure 2 Histopathological features of the lesion. Small lymphoid cells are proliferative at the superficial dermis and the perifollicular areas.
Figure 3 Immunohistochemical analysis via the avidin‐biotin‐peroxidase complex method. Dense infiltration of CD4‐positive small lymphoid cells is evident at the superficial dermis. Bar = 200 μm.